|
KMID : 0613820120220040447
|
|
Journal of Life Science
2012 Volume.22 No. 4 p.447 ~ p.455
|
|
|
Regulation of Skeletal Muscle Differentiation by Akt
|
|
Woo Dae-Han
Yun Sung-Ji
Kim Eun-Kyoung
Ha Jung-Min
Shin Hwa-Kyoung
Bae Sun-Sik
|
|
|
Abstract
|
|
|
|
Akt plays an important role in a variety of cellular physiologies such as growth, proliferation, and differentiation. In skeletal muscle, Akt has been implicated in regulating regeneration, hypertrophy, and atrophy. In this study, the role of Akt has been examined during skeletal muscle differentiation. Culturing C2C12 myoblasts under low serum (1% horse serum) and high density converted cell morphology from a round shape to an elongated and multi-nucleated shape. Morphological changes were initiated from day 2 of differentiation. In addition, the expression of both myogenin G and myogenin D was elevated from day 2 of differentiation. Skeletal muscle differentiation was abolished by silencing Akt1 or Akt2, but was significantly enhanced by the over-expression of either Akt1 or Akt2. The activation of Akt was observed from day 2 of differentiation and disappeared after day 7. The expression of kruppel-like factor 4 was observed from day 6 of differentiation. Moreover, this expression was blocked in cells silencing either Akt1 or Akt2. In addition, the promoter activity of kruppel-like factor 4 was significantly reduced in cells silencing Akt1 or Akt2. These results suggest that Akt regulates skeletal muscle differentiation through the regulation of kruppel-like factor 4 expression.
|
|
|
KEYWORD
|
|
|
Muscle, differentiation, Akt, KLF4, myogenin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|